The Kinetic Models of the Bioprocess with Free and Immobilized Cells

The kinetic models are fundamental knowledge about biochemical and microbial processes. The object of the work is the kinetic model researching based on the biochemical and microbial mechanisms of cell growth, substrate consumption and product formation. The ethanol biosynthesis process as fuel from renewable resources is the engineering goal. The engineering goal requires the inclusion of the inhibitory effect of high substrate and ethanol concentration in the kinetic models. The interactions of the ethanol synthesis with growth biomass mechanism were researched. The models of the ethanol forming mechanisms were incorporated in the complex structure of the kinetic models. The researches of the kinetic models were realized by computer simulation and experimental verification at the ethanol biosynthesis process from glucose with free and immobilized cells S. cerevisiae. The model parameters and limits were estimated by experimental validation. The base of the verified deterministic and semi-empiric kinetic models was created. The model base may be onward used for the process simulation models generating.